Lindahl, Johanna
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- International Livestock Research Institute (ILRI)
- Uppsala University
Abstract
Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens.
Keywords
Serology; Arbovirus; Vector-borne disease; DIVA test; Seroconversion
Published in
Journal of Virological Methods
2019, volume: 269, pages: 70-76
Publisher: ELSEVIER SCIENCE BV
SLU Authors
UKÄ Subject classification
Pathobiology
Publication identifier
- DOI: https://doi.org/10.1016/j.jviromet.2019.04.011
Permanent link to this page (URI)
https://res.slu.se/id/publ/100647