Terenius, Olle
- Department of Ecology, Swedish University of Agricultural Sciences
Abstract
Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU were examined using light microscopy and scanning electron microscopy. The polyhedral inclusion bodies (PIBs) of BmNPV-MSU appeared in tetragonal, hexagonal, octagonal, and globular forms. The virions were both single and multiple embedded as observed by transmission electron microscopy. We also determined the virulence of BmNPV-MSU for six different Thai polyvoltine strains by LC50 and time to death after infection. The LC50 values of Nang Lai, NK04, and Sam Rong strains were 5.05-1.52 x 10(7) PIBs per ml and mortality peaked 7- to 8-d after inoculation. For Nang Noi, SP2, and RE05 strains the LC50 values were 7.91-1.82 x 10(6) PIBs/ml and mortality peaked 4-5 d after inoculation, thus having lower chance of survival to infection by BmNPV-MSU.
Keywords
polyvoltine; Bombyx mori; nucleopolyhedrovirus; BmNPV-MSU; virulence
Published in
Journal Of Insect Science (Online)
2020, volume: 20, number: 2, article number: ieaa023
Publisher: OXFORD UNIV PRESS INC
SLU Authors
UKÄ Subject classification
Microbiology
Publication identifier
- DOI: https://doi.org/10.1093/jisesa/ieaa023
Permanent link to this page (URI)
https://res.slu.se/id/publ/105589