An efficient protocol for genetic transformation and shoot regeneration of turmeric (Curcuma longa L.) via particle bombardment

Abstract

Turmeric (Curcuma longa L.) is an important spice crop plant that is sterile and cannot be improved by conventional breeding. An efficient method for stable transformation for turmeric, C. longa L., was developed using particle bombardment. Callus cultures initiated from shoots were bombarded with gold particles coated with plasmid pAHC25 containing the bar and gusA genes each driven by the maize ubiquitin promoter. Transformants were selected on medium containing glufosinate. Transgenic lines were established on selection medium from 50% of the bombarded calluses. Transgenic shoots regenerated from these were multiplied and stably transformed plantlets were produced. Polymerase chain reaction (PCR) and histochemical GUS assay confirmed the stable transformation. Transformed plantlets were resistant to glufosinate

Published in

Plant Cell Reports
2006, volume: 25, number: 2, pages: 112-116
Publisher: SPRINGER

SLU Authors

• Clapham, David

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Von Arnold, Sara

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

Publication identifier

• DOI: https://doi.org/10.1007/s00299-005-0033-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/10584