Natural variation of root lesion nematode antagonism in the biocontrol fungus Clonostachys rosea and identification of biocontrol factors through genome-wide association mapping

Abstract

Biological control is a promising approach to reduce plant diseases caused by nematodes to ensure high productivity in agricultural production. Large-scale analyses of genetic variation in fungal species used for biocontrol can generate knowledge regarding interaction mechanisms that can improve efficacy of biocontrol applications. In this study, we performed a genome-wide association study (GWAS) for in vitro antagonism against the root lesion nematode Pratylenchus penetrans in 53 previously genome re-sequenced strains of the biocontrol fungus Clonostachys rosea. Nematode mortality in C. rosea potato dextrose broth (PDB) culture filtrates was highly variable and showed continuous variation (p < .001) between strains, indicating a polygenic inheritance. Twenty-one strains produced culture filtrates with higher (p

Keywords

antagonism; biocontrol; Clonostachys rosea; genome-wide association study; phytopathogenic nematodes; plant growth; wheat

Published in

Evolutionary applications
2020, volume: 13, number: 9, pages: 2264-2283

SLU Authors

• Iqbal, Mudassir

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Broberg, Martin

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Broberg, Anders

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Brandström Durling, Mikael

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Viketoft, Maria

  • Department of Ecology, Swedish University of Agricultural Sciences
    

• Jensen, Dan Funck

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Dubey, Mukesh

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Karlsson, Magnus

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network

UKÄ Subject classification

Agricultural Science
Ecology

Publication identifier

• DOI: https://doi.org/10.1111/eva.13001

Permanent link to this page (URI)

https://res.slu.se/id/publ/106040