LPS-treatment of bovine endometrial epithelial cells causes differential DNA methylation of genes associated with inflammation and endometrial function

Abstract

BackgroundLipopolysaccharide (LPS) endotoxin stimulates pro-inflammatory pathways and is a key player in the pathological mechanisms involved in the development of endometritis. This study aimed to investigate LPS-induced DNA methylation changes in bovine endometrial epithelial cells (bEECs), which may affect endometrial function. Following in vitro culture, bEECs from three cows were either untreated (0) or exposed to 2 and 8 mu g/mL LPS for 24h.ResultsDNA samples extracted at 0h and 24h were sequenced using reduced representation bisulfite sequencing (RRBS). When comparing DNA methylation results at 24h to time 0h, a larger proportion of hypomethylated regions were identified in the LPS-treated groups, whereas the trend was opposite in controls. When comparing LPS groups to controls at 24h, a total of 1291 differentially methylated regions (DMRs) were identified (55% hypomethylated and 45% hypermethylated). Integration of DNA methylation data obtained here with our previously published gene expression data obtained from the same samples showed a negative correlation (r=-0.41 for gene promoter, r=-0.22 for gene body regions, p

Keywords

LPS; Bovine endometrial epithelial cells; RRBS; Endometritis; Inflammation; Implantation

Published in

BMC Genomics
2020, volume: 21, number: 1
Publisher: BMC

SLU Authors

• Jhamat, Naveed

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • University of the Punjab

• Niazi, Adnan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Guo, Yongzhi

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Chanrot, Metasu

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Rajamangala University of Technology

• Bongcam Rudloff, Erik

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Andersson, Göran

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Humblot, Patrice

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics and Breeding in Agricultural Sciences

Publication identifier

• DOI: https://doi.org/10.1186/s12864-020-06777-7

Permanent link to this page (URI)

https://res.slu.se/id/publ/106776