Meschichi, Anis
- Department of Plant Biology, Swedish University of Agricultural Sciences
Abstract
In eukaryotes, DNA is packed into an incredibly complex structure called chromatin. Although chromatinwas often considered as a static entity, it is now clear that chromatin proteins and the chromatin fiber itselfare in fact very dynamic. For instance, the packaging of the DNA into the nucleus requires an extraordinarydegree of compaction but this should be achieved without compromising the accessibility to the transcriptionmachinery and other nuclear processes. Approaches such as gene tagging have been established forliving cells in order to detect, track, and analyze the mobility of single loci. In this chapter, we provide anexperimental protocol for performing locus tracking in Arabidopsis thaliana roots and for characterizinglocus mobility behavior via a Mean Square Displacement analysis.
Published in
Methods in Molecular Biology
2021, volume: 2200, number: volume 2200, pages: 213-224
Title: Arabidopsis protocols
Publisher: Springer Nature
SLU Authors
Rosa, Stefanie
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Biochemistry
Molecular Biology
Publication identifier
- DOI: https://doi.org/10.1007/978-1-0716-0880-7_10
- ISBN: 978-1-0716-0879-1
- eISBN: 978-1-0716-0880-7
Permanent link to this page (URI)
https://res.slu.se/id/publ/108799