The metabolic performance and microbial communities of anaerobic digestion of chicken manure under stressed ammonia condition: A case study of a 10-year successful biogas plant

Abstract

To increase the knowledge of microbial adaptation for high degradation ability under ammonia stressed conditions, the metabolic performance and the microbial community in a full-scale chicken manure anaerobic digestion (AD) plant, operated over a 10-year, were investigated. The methanogenic pathway employing labeled acetate (2-C-13), aceticlastic methanogenesis activity, and the Illumina sequencing of microbiome structure at the plant were analyzed. The obtained results show that this active plant achieved a methane yield of 310 +/- 43 mL/g-volatile solids (VS), high stability under an ammonium-N level of 6.2 +/- 0.1 g/L, and an organic loading rate of 2.5 g-VS/(L.d). The extremely long adaptation developed the unique microbe communities, and the hydrogentrophic methanogens Methanobrevibacter (76%) and Methanoculleus (18%) were dominant in the main digester. The acetoclastic methanogenic pathway reached 42%, contributing to the deep degradation. The current study thus may lay the foundation for illustrating the successful full-scale AD of nitrogen-rich materials under stressed operating conditions. (c) 2020 Elsevier Ltd. All rights reserved.

Keywords

Anaerobic digestion; Full-scale biogas plant; Microbial acclimation; Methanogenic pathway; Microbial communities; Chicken manure

Published in

Renewable Energy
2021, volume: 167, pages: 644-651
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

• Westerholm, Maria

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

Global goals (SDG)

SDG9 Industry, innovation and infrastructure

UKÄ Subject classification

Microbiology
Bioenergy

Publication identifier

• DOI: https://doi.org/10.1016/j.renene.2020.11.133

Permanent link to this page (URI)

https://res.slu.se/id/publ/110504