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Abstract

Agro-industrial wastes suitable for economical and high mass production of novel Trichoderma species under solid-state fermentation were identified by optimizing the culture conditions using a mathematical model and evaluating the viability of the formulated bio-product. Fourteen inexpensive, locally available, organic substrates and cereals were examined using a one-factor-at-a-time experiment. The fungus colonized nearly all substrates after 21 days of incubation, although the degree of colonization and conidiation varied among the substrates. A mixture of wheat bran and white rice (2:1 w/w) was found to support maximum growth of T. asperellum AU131 (3.2 x 10(7) spores/g dry substrate) and T. longibrachiatum AU158 (3.5 x 10(7) spores/g dry substrate). Using a fractional factorial design, the most significant growth factors influencing biomass production were found to be temperature, moisture content, inoculum concentration, and incubation period (p

Keywords

agro-industrial waste; bioformulation; response surface methodology; viability; wheat bran

Published in

Microorganisms
2021, volume: 9, number: 8, article number: 1675
Publisher: MDPI

SLU Authors

UKÄ Subject classification

Chemical Engineering

Publication identifier

  • DOI: https://doi.org/10.3390/microorganisms9081675

Permanent link to this page (URI)

https://res.slu.se/id/publ/113546