Cojkic, Aleksandar
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Abstract
Antimicrobial agents are added to semen extenders to prevent growth of bacteria in semen. The aim of this study was to evaluate the antimicrobial potential of fresh pomegranate juice (PJ) and reconstituted commercial pomegranate powder (PP), on the microbiota collected from bull semen. Bacterial broth (BB) was made from 25 bacterial strains isolated from semen of one bull. The treatments were created using three ratios of BB and PJ (1:0.5; 1:1; 1:2), in addition to BB and PP (1:1); three concentrations of PP were assessed (5%, 10%, 20%). The PP and PJ were filtered to exclude bacterial contamination. Bacterial growth was determined after 0 and 24 hours of exposure at room temperature. Antimicrobial efficiency was determined from bacterial counts (logarithmic colony-forming units; CFU per mL) before and after incubation for 72 hours at 30°C. The experiment was repeated three times. Multiple T test and ANOVA Dunnett`s multiple comparison test (GraphPadPrisma 7.00) were performed to analyse the date. Confidence interval P
Keywords
Punica granatum; bull semen microbiota; antimicrobial effect
Published in
Title: Book of abstracts : XII international scientific agriculture symposium “AGROSYM 2021”
Publisher: AgroSym
Conference
XII International scientific agriculture symposium “AGROSYM 2021”, 211007-211010, Jahorina, Bosnia and Hercegovina
SLU Authors
Hansson, Ingrid
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Animal and Dairy Science
Publication identifier
- ISBN: 978-99976-787-8-2
Permanent link to this page (URI)
https://res.slu.se/id/publ/114544