Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease

Abstract

Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein beta-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-beta-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.

Keywords

climate change; disease control; host parasite interactions; oomycetes; pest management

Published in

Phytopathology
2021, volume: 111, number: 12, pages: 2168-2175

SLU Authors

• Kalyandurg, Pruthvi Balachandra

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Sundararajan, Poorva

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Dubey, Mukesh

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Ghadamgahi, Farideh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    
  • Ferdowsi University of Mashhad

• Zahid, Muhammad Awais

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Vetukuri, Ramesh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

Associated SLU-program

AMR: Fungus

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1094/PHYTO-02-21-0054-SC

Permanent link to this page (URI)

https://res.slu.se/id/publ/114915