Gröndahl, Gittan
- Department of Large Animal Clinical Sciences, Swedish University of Agricultural Sciences
Abstract
Infectious diseases are the main cause of disease and deaths in foals. The neutrophil phagocyte is a part of the early, non-specific immune response, which is essential in defending the host against microbial invasion. Coating of microbes with opsonic factors such as antibodies and complement from serum is required for optimal phagocytosis.Flow cytometric methods were developed and used for studies of equine neutrophil phagocytosis and serum opsonisation of Saccharomyces cerevisiae, Escherichia coli and Actinobacillus equuli, and of the expression of complement receptor subunit CD18 on neutrophils. Foals from birth to 2 months of age and adult horses were studied.Incubation for 15 min was found sufficient for both opsonisation and phagocytosis. Opsonisation of yeast and A. equuli, but not of E. coli, was observed already at low serum concentrations (
Keywords
neutrophil; phagocytosis; opsonisation; foals; complement; IgG; transfusion; Escherichia coli; Actinobacillus equuli; colostrum; flow cytometry
Published in
Acta Universitatis Agriculturae Sueciae. Veterinaria
2001, number: 105
Publisher: Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Clinical Science
Publication identifier
- ISBN: 91-576-5931-1
Permanent link to this page (URI)
https://res.slu.se/id/publ/117487