Sharma, Shrikant
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Research article2023Peer reviewedOpen access
Pho1a (plastid starch phosphorylase) is duplicated and essential for normal starch granule phenotype in tubers of Solanum tuberosum L
Sharma, Shrikant; Friberg, Martin; Vogel, Paul; Turesson, Helle; Olsson, Niklas; Andersson, Mariette; Hofvander, Per
Abstract
Reserve starch from seeds and tubers is a crucial plant product for human survival. Much research has been devoted to quantitative and qualitative aspects of starch synthesis and its relation to abiotic factors of importance in agriculture. Certain aspects of genetic factors and enzymes influencing carbon assimilation into starch granules remain elusive after many decades of research. Starch phosphorylase (Pho) can operate, depending on metabolic conditions, in a synthetic and degradative pathway. The plastidial form of the enzyme is one of the most highly expressed genes in potato tubers, and the encoded product is imported into starch-synthesizing amyloplasts. We identified that the genomic locus of a Pho1a-type starch phosphorylase is duplicated in potato. Our study further shows that the enzyme is of importance for a normal starch granule phenotype in tubers. Null mutants created by genome editing display rounded starch granules in an increased number that contained a reduced ratio of apparent amylose in the starch.
Keywords
starch phosphorylase; reserve starch; sink tissue; starch granule; tuber; potato; CRISPR; gene editing
Published in
Frontiers in Plant Science
2023, volume: 14, article number: 1220973
Publisher: FRONTIERS MEDIA SA
SLU Authors
Friberg, Martin
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Vogel, Paul
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Turesson, Helle
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Olsson, Niklas
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Andersson, Mariette
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Hofvander, Per
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.3389/fpls.2023.1220973
Permanent link to this page (URI)
https://res.slu.se/id/publ/124690