Svenson, Stefan
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
We evaluated the random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) techniques for studying an outbreak of beta-haemolytic streptococci group A (GAS) occurred at two maternity wards at Danderyd hospital, Stockholm, Sweden. All the isolates were of T-type 8,25. The RAPD technique revealed that all RAPD-PCR profiles were identical. PFGE showed that all the patterns but one were identical. These patterns were compared with 10 different T-type GAS from the strain collection of the Swedish Institute for Infectious Disease Control (SMI) and T-type 8,25 from different years and locations. The SMI strains exhibited patterns different from each other and all different from the isolates from Danderyd hospital. Moreover, RAPD could not differentiate among the T-type 8,25 isolates from different years and locations but PFGE showed differences among the amplicons. Our results indicated that the RAPD and PFGE techniques could be efficient tools in epidemiological studies of GAS. (C) 2002 Elsevier Science Ltd. All rights reserved
Published in
Comparative Immunology, Microbiology and Infectious Diseases
2003, volume: 26, number: 1, pages: 25-35
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
SLU Authors
Gonzalez Rey, Carlos
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Krovacek, Karel
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/S0147-9571(02)00019-X
Permanent link to this page (URI)
https://res.slu.se/id/publ/1266