Johannisson, Anders
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Abstract
Equine breeding would benefit greatly from reliable biomarkers of stallion or ejaculate fertility. The aim of the study was to investigate how several in vitro sperm characteristics correlate with fertility after artificial insemination, to explore the potential to build a fertility prediction model for stallions. Cooled insemination doses (3-5 per stallion) were obtained from various studs. Sperm membrane integrity, acrosome integrity, chromatin integrity, mitochondrial membrane potential, and reactive oxygen species production were evaluated by flow cytometry 24-30 h after semen collection, and sperm motility was assessed by computer aided sperm analysis. Calcein violet was used to differentiate viable spermatozoa. Per season pregnancy rates for these stallions were available the following year. Positive correlations were found between pregnancy rate and straightness (r = 0.43, p
Keywords
Flow-cytometric analysis; Sperm kinematics; Artificial insemination
Published in
Veterinary Research Communications
2024, volume: 48, number: 4, pages: 2157–2169
Publisher: SPRINGER
SLU Authors
Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Ntallaris, Theodoros
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Clinical Science
Publication identifier
- DOI: https://doi.org/10.1007/s11259-024-10372-6
Permanent link to this page (URI)
https://res.slu.se/id/publ/129411