New insights into the mechanisms of plant isotope fractionation from combined analysis of intramolecular 13C and deuterium abundances in Pinus nigra tree-ring glucose

Abstract

Understanding isotope fractionation mechanisms is fundamental for analyses of plant ecophysiology and paleoclimate based on tree-ring isotope data. To gain new insights into isotope fractionation, we analysed intramolecular C-13 discrimination in tree-ring glucose (Delta(i)', i = C-1 to C-6) and metabolic deuterium fractionation at H-1 and H-2 (epsilon(met)) combinedly. This dual-isotope approach was used for isotope-signal deconvolution. We found evidence for metabolic processes affecting Delta(1)' and Delta(3)', which respond to air vapour pressure deficit (VPD), and processes affecting Delta(1)', Delta(2)', and epsilon(met), which respond to precipitation but not VPD. These relationships exhibit change points dividing a period of homeostasis (1961-1980) from a period of metabolic adjustment (1983-1995). Homeostasis may result from sufficient groundwater availability. Additionally, we found Delta(5)' and Delta(6)' relationships with radiation and temperature, which are temporally stable and consistent with previously proposed isotope fractionation mechanisms. Based on the multitude of climate covariables, intramolecular carbon isotope analysis has a remarkable potential for climate reconstruction. While isotope fractionation beyond leaves is currently considered to be constant, we propose significant parts of the carbon and hydrogen isotope variation in tree-ring glucose originate in stems (precipitation-dependent signals). As basis for follow-up studies, we propose mechanisms introducing Delta(1)', Delta(2)', Delta(3)', and epsilon(met) variability.

Keywords

carbon stable isotopes; hydrogen stable isotopes; intramolecular isotope analysis; isotope fractionation mechanisms; leaf water status; plant-environment interactions; stem water status; tree rings

Published in

New Phytologist
2025, volume: 245, number: 3, pages: 1000-1017
Publisher: WILEY

SLU Authors

• Wieloch, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • California Institute of Technology

• Niittylä, Totte

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany

Publication identifier

• DOI: https://doi.org/10.1111/nph.20113

Permanent link to this page (URI)

https://res.slu.se/id/publ/132712