Chen, Feng
- Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
- Anhui Agricultural University (AAU)
Research article2025Peer reviewedOpen access
Mild thermal treatment assists fungal preprocessing of softwood sawdust for production of fermentable sugar
Chen, Feng; Martin, Carlos; Jonsson, Leif J.; Gandla, Madhavi Latha; Klausen, Sarah J.; Soto, Luis A. Romero; Xiong, Shaojun
Abstract
Preheating with hot air at 85 - 125 degrees C was evaluated for its effectiveness in removing terpenes and terpenoids in softwood sawdust, thereby enhancing fungal preprocessing and subsequent saccharification of softwood-based mushroom substrates. Sawdust from pine (Pinus sylvestris L.) and spruce (Picea abies (L.) H. Karst.) was preheated prior to shiitake (Lentinula edodes (Berk.) Pegler) cultivation. Preheating removed up to 96 % of terpenes in pine- based substrates and up to 50 % in spruce-based substrates. Additionally, preheating decreased total terpenoids content in spruce by up to 78 %. For the pine-based substrate, the mild heating generally led to faster colonisation and improved mushroom yield, with the fastest mycelia colonisation and highest yield observed for 105 degrees C treatment. This temperature was associated with the lowest content of total terpenes and absence of major monoterpenes. The content of terpenes and terpenoids continued to decrease during cultivation, alongside fungal degradation of lignocellulose. As a result of more extensive lignin degradation, the enzymatic digestibility of cellulose was higher for spruce-based spent mushroom substrate than for pine-based one (up to 89 % vs. 49 % conversion). Enzymatic digestibility showed a negative correlation with the alpha-pinene content, and a positive correlation with increasing preheating temperatures.
Keywords
Shiitake mushroom; Fungal pretreatment; Lignocellulose; Terpenes; Enzymatic saccharification; Biorefinery
Published in
Industrial Crops and Products
2025, volume: 223, article number: 120284
Publisher: ELSEVIER
SLU Authors
Xiong, Shaojun
- Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
- Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Biocatalysis and Enzyme Technology
Publication identifier
- DOI: https://doi.org/10.1016/j.indcrop.2024.120284
Permanent link to this page (URI)
https://res.slu.se/id/publ/140675