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Abstract

This study investigated the protective effects of Pandanus tectorius leaf extract (PLE) on the tolerance of the brine shrimp Artemia franciscana against the pathogen Vibrio campbellii. Axenic Artemia nauplii were hatched and exposed to PLE for 2 h, after which their resistance to V. campbellii was examined. Protective responses were evaluated by measuring nauplii survival and changes in the expression of key immune-related genes, such as heat shock protein 70 (hsp70), hsp60, hsp90, high mobility group box 1 (hmgb1), prophenoloxidase (proPO), and transglutaminase (tgase) upon V. campbellii challenge. Additionally, a Vibrio colonization assay was conducted to assess PLE's antimicrobial potential. The results revealed that 2 h pretreatment with PLE at a concentration of 1 g/L significantly improved survival against V. campbellii. Immune-related gene expression was induced at different time points (e.g., 12-24 h) during the Vibrio challenge and the colonization assay confirmed PLE's antimicrobial properties. The observed protection could likely be due to a combination of immune gene activation, antioxidant activity, and the antimicrobial effect of the extract. This study highlights the significance of PLE by indicating its potential to serve as a protective agent in aquaculture, enhancing the resilience of aquatic organisms against biotic stressors like vibrios.

Keywords

antimicrobial properties; antioxidant properties; axenic Artemia nauplii; immune-related genes; Pandanus tectorius leaf extract; V. campbellii

Published in

Aquaculture Research
2025, volume: 2025, number: 1, article number: 8215825
Publisher: WILEY

SLU Authors

UKÄ Subject classification

Pathobiology
Fish and Aquacultural Science

Publication identifier

  • DOI: https://doi.org/10.1155/are/8215825

Permanent link to this page (URI)

https://res.slu.se/id/publ/141738