Ingvarsson, Pär
- Department of Plant Biology, Swedish University of Agricultural Sciences
Abstract
Pathogenic microorganisms cause infectious plant diseases, which limit crop productivity and cause economic losses. Early detection enables improved pathogen recognition and more effective control through integrated pest management strategies. Burkholderia gladioli pv. gladioli (Bgg) cause saffron bacterial corm rot (BCR), a hardto-detect disease, which has a devastating effect with up to 80 % field destruction rates and corresponding heavy yield losses. In this study, (two isolates of Bgg (A and B)) were identified via TEM and SEM image analysis followed by molecular identification by 23S rRNA PCR. A Bgg-specific polyclonal antibody was developed and was conjugated to alkaline phosphatase for direct assays, and no conjugation was used for indirect assays employing a secondary antibody. ELISA, Western blot, dot blot and immunofluorescence imaging were used to evaluate the efficacy and specificity of the developed antibody. The raised antibody bound specifically to Bgg with very low cross-reactivities to structurally related bacteria. Dot blot was shown as a suitable, cheap, and rapid field test. This work represents an important step forward to diagnose and prevent the spread of BCR.
Keywords
Crocus sativus; Dot blot; Electron microscopy; ELISA; Immunofluorescence; Polyclonal antibody
Published in
Journal of Agriculture and Food Research
2025, volume: 23, article number: 102281
Publisher: ELSEVIER
SLU Authors
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1016/j.jafr.2025.102281
Permanent link to this page (URI)
https://res.slu.se/id/publ/144439