Uggla, Arvid
- Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences
Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Neospora caninum in serum from cattle is described. Extracted tachyzoite proteins incorporated into immunostimulating complexes (iscoms) were used as coating antigen and a mouse monoclonal antibody to bovine immunoglobulin G1 as conjugate. Western blot analysis of the iscom preparation revealed a restricted number of antigens compared with whole parasite homogenates. When probed with a serum from an experimentally infected calf, heavily stained antigens with apparent molecular masses of 28, 35, 45 and 78 kDa were seen. The sensitivity and specificity of the ELISA was 100% and 96%, respectively, against an indirect fluorescent antibody test as indicator of true status. The applicability of the ELISA for demonstration of antibodies in milk was evaluated and the agreement between serum and milk ELISA was 95%.
Keywords
Neospora caninum; cattle-protozoa; antibodies; ELISA; iscom
Published in
Veterinary Parasitology
1997, volume: 68, number: 3, pages: 251-260
SLU Authors
UKÄ Subject classification
Animal and Dairy Science
Clinical Science
Publication identifier
- DOI: https://doi.org/10.1016/S0304-4017(96)01076-X
Permanent link to this page (URI)
https://res.slu.se/id/publ/145147