Daniel, Geoffrey
- Department of Forest Products, Swedish University of Agricultural Sciences
Abstract
The effect of chitosan on cell wall deposition was investigated in the two wood-inhabiting fungal species Trichoderma harzianum (CBS 597.91) and Sphaeropsis sapinea (NZFS 2725). The study used three independent analytical techniques to quantify chitin in the fungal mycelium. A colorimetric method for the detection of D-glucosamine was compared with two gas chromatography-mass spectroscopy (GC-MS) methods employing alditol acetates analysis and pyrolysis. The latter used a stable-isotope-labelled internal standard, d(3)-N-acetyl glucosamine. At least in the case of S. sapinea, the study provided evidence of an increase in the chitin content in the mycelium due to chitosan treatment, indicating that chitosan treatment affected cell wall deposition. Electron microscopy techniques showed alteration in surface morphology and cell wall texture due to chitosan treatment. The implications of these results are discussed with a view to analysing possible mechanisms for growth inhibitory effects of chitosan on fungal hyphae. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Keywords
alditol acetates; chitin determination; colorimetric method; electron microscopy; GC-MS; pyrolysis; Sphaeropsis sapinea; Trichoderma harzianum
Published in
Mycological Research
2007, volume: 111, pages: 875-890
Publisher: ELSEVIER SCI LTD
SLU Authors
UKÄ Subject classification
Forest Science
Publication identifier
- DOI: https://doi.org/10.1016/j.mycres.2007.05.015
Permanent link to this page (URI)
https://res.slu.se/id/publ/14935