Pettersson, Hans
- Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
Abstract
Commercially available solid zearalenone (ZON) to be used as a certified liquid calibrant (BCR-699) in a project funded by the European Commission within the Standard Measurement and Testing program was characterized and its purity determined. The degree of purity of the ZON was examined by UV spectrophotometer, liquid chromatography (LC) with diode array and fluorescence detection, H-1 and C-13-NMR spectrometry, LC-mass spectrometry (LC/MS/MS), ion chromatography (IC), and differential scanning calorimetry (DSC). The diagrams obtained from DSC analysis and the UV spectrum showed no detectable impurities. Likewise, no impurities were observed by LC analysis with both diode array and fluorescence detection. IC determination revealed negligible contamination of ZON with chloride of 0.020 +/- 0.005 % and nitrate of 0.016 +/- 0.006%. Zearalanone (ZAN) was identified as one of 2 minor (0.2%) impurities by LC/MS/MS. The H-1-NMR measurements revealed an additional peak, which has not been previously reported in the literature. It could be identified as part of the ZON spectrum as the signal arising from the phenolic proton attached to C-4. The manufacturer states an additional contamination with 0.2% methylene chloride, which could be confirmed to an extent of 0.1 % by 1H-NMR. Minor impurities, whose structures remain unknown, were discovered at 3.5 and
Keywords
Mycotoxin Zearalenone; Estrogenic Mycotoxins; Physicochemical Data; Deoxynivalenol; Toxins
Published in
Journal of AOAC INTERNATIONAL
2003, volume: 86, number: 4, pages: 722-728
Publisher: AOAC INTERNATIONAL
SLU Authors
UKÄ Subject classification
Veterinary Science
Food Science
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1093/jaoac/86.4.722
Permanent link to this page (URI)
https://res.slu.se/id/publ/1508