Belak, Sandor
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
The emergence and re-emergence of transboundary animal diseases (TADs), e.g., foot-and-mouth disease, classical swine fever and the highly pathogenic avian influenza strongly indicate the need for the development of powerful and robust new diagnostic methods. The experiences of an OIE-Collaborating Centre and of two EU project consortia are summarised on the diagnostic application of gel-based PCR, general PCR systems, phylogeny, molecular epidemiology, real-time PCR (TaqMan, Molecular Beacons, Primer-Probe Energy Transfer), amplification without thermocycling (Invader), multiplex PCR, nucleic acid extraction and pipetting robotics, automation and quality control, including internal controls. By following the steps of OIE validation, the diagnostic assays are nationally and internationally standardised. The development of padlock probes and microarrays, as well as ultra rapid PCR and sequencing methods is further improving the arsenal of nucleic acid based molecular diagnosis. Further trends of diagnostic development are also mentioned, in order to combat TADs and other viral infections more effectively in the future. (c) 2006 Elsevier Ltd. All rights reserved.
Keywords
molecular diagnosis; PCR; automation; validation; padlock probes; microarrays
Published in
Vaccine
2007, volume: 25, number: 30, pages: 5444-5452
Publisher: ELSEVIER SCI LTD
SLU Authors
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1016/j.vaccine.2006.11.068
Permanent link to this page (URI)
https://res.slu.se/id/publ/15106