Broberg, Anders
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
The feasibility of using coumarin-3-carboxylic acid (3-CCA) for detection of hydroxyl radicals in pure cultures of wood-decaying fungi was tested. Fungi were incubated on a 3-CCA-containing medium. The transformation of 3-CCA to the fluorescent hydroxyl radical detector substance 7-hydroxycoumarin-3-carboxylic acid and other compounds was studied by chromatographic and spectroscopic techniques. During incubation of all tested fungi, a small fraction of the 3-CCA was hydroxylated to 7-hydroxycoumarin-3-carboxylic acid and a major fraction of the 3-CCA was metabolized by fungi to 2-(2-hydroxybenzyl)malonic acid. In most cultures the concentration of 3-CCA was below detection limit at the end of incubation. The fungal metabolism was suggested to be involved in the formation of 2-(2-hydroxybenzvl)malonic acid from 3-CCA, consequently making this method of hydroxyl radical detection less suitable to use on cultures of wood-decaying fungi. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved
Keywords
Hydroxyl radical detection; Coumarin-3-carboxylic acid; 7-hydroxycoumarin-3-carboxylic acid; Fungal physiology
Published in
FEMS Microbiology Ecology
2003, volume: 46, number: 2, pages: 197-202
Publisher: ELSEVIER SCIENCE BV
SLU Authors
Stenlid, Jan
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Subatomic Physics
Publication identifier
- DOI: https://doi.org/10.1016/S0168-6496(03)00213-7
Permanent link to this page (URI)
https://res.slu.se/id/publ/17