Johansson, Monika
- Department of Plant Biology, Swedish University of Agricultural Sciences
Abstract
The enzyme nucleoside diphosphate kinase (NDPK) is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions. Here we present evidence of an interaction between the plant mitochondrial NDPK and a plant mitochondrial adenylate kinase. This is the most highly expressed adenylate kinase gene present in Arabidopsis thaliana. The adenylate kinase cDNA was amplified from A. thaliana and cloned into an expression vector. The recombinant His-tagged protein was produced in Escherichia coli and purified. In vitro experiments using the recombinant enzymes showed that NDPK and adenylate kinase affected the activity of each other. Adenylate kinase stimulated NDPK activity, whereas NDPK inhibited the activity of adenylate kinase, when the total ADP production of the enzymes was measured. The net effect could be an unchanged ADP production, albeit with different rates of substrate consumption. Possible implications in respiration and programmed cell death are discussed. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
Keywords
NDPK; adenylate kinase; mitochondria; plant; inter membrane space
Published in
Plant Science
2008, volume: 174, number: 2, pages: 192-199
Publisher: ELSEVIER IRELAND LTD
SLU Authors
Priddle, Jenni
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
Knorpp, Carina
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.plantsci.2007.11.005
Permanent link to this page (URI)
https://res.slu.se/id/publ/20401