Tigabu, Mulualem
- Department of Silviculture, Swedish University of Agricultural Sciences
Near infrared spectroscopy was used for simultaneous detection of filled, empty and insect-infested seeds of Larix decidua Mill., Larix gmelinii Rupr. and Larix sukaczewii Dyl. Near-infrared spectra, expressed in the form of log (1/T), were collected from single seeds with 1225 Infratec analyzer from 850 - 1048 nm at 2 nm interval. Discriminant models were developed with partial least squares (PLS) regression using the normalized digital spectra as a regressor and a y-vector of artificial values ( 1 for filled and 21 for empty and infested seeds) as a regressand. The result showed a 100% recognition of infested and empty seeds in the test set by 3-factor PLS models computed based on calibration sets of each species and combined spectra of all species. The recognition rate of filled seeds was 90 - 100%; the highest being for L. sukaczewii, followed by L. decidua and L. gmelinii respectively. A similar recognition rate for empty and infested seeds was achieved by discriminant models derived from selected absorption bands, while the recognition rate of filled seeds differed slightly. Difference spectra and PLS weights indicated that the origin of spectral differences between filled versus empty and insect-infested seeds were attributed to differences in lipids and proteins.
European larch; NIR; NIRS; Siberian larch; tree seed quality
New Forests
2004, volume: 27, number: 1, pages: 39-53
Publisher: KLUWER ACADEMIC PUBL
Forest Science
https://res.slu.se/id/publ/2989