Typing of Brachyspira spp. from rodents, pigs and chickens on Swedish farms

Abstract

The aim of the current study was to look for evidence of possible cross-species transmission of Brachyspira species between rodents and farm animals. To do this, previously collected and characterised Brachyspira isolates from rodents, pigs and chickens on the same farms were analysed by random amplified polymorphic DNA (RAPD). Isolates with similar RAPD banding patterns were further typed by pulsed-field gel electrophoresis (PFGE). Identical isolates of Brachyspira pilosicoli, Brachyspira intermedia, Brachyspira murdochii and Brachyspira innocens from pigs and rodents and of B. murdochii from laying hens and rodents were found, indicating cross-species transmission at farm level. PFGE data from rodent isolates of Brachyspira hyodysenteriae were compared with PFGE data from previously typed field isolates of B. hyodysenteriae from pigs with swine dysentery and isolates from mallards (Anas platyrhynchos). Three of four isolates of B. hyodysenteriae from rodents were similar to porcine field isolates by PFGE. PCR analyses of the plasmid-encoded and potential virulence determinants rib genes B, A, D and C showed that they were present in isolates of B. hyodysenteriae of porcine, mallard and rodent origin. (C) 2011 Elsevier B.V. All rights reserved.

Keywords

Brachyspira; Rodent; PFGE; RAPD; rfbBADC

Published in

Veterinary Microbiology
2011, volume: 153, number: 1-2, pages: 156-162

SLU Authors

• Backhans, Annette

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Aspán, Anna

  • National Veterinary Institute (SVA)

• Fellström, Claes

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1016/j.vetmic.2011.03.023

Permanent link to this page (URI)

https://res.slu.se/id/publ/35025