Nalawade, Satish
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Mahyco Research Center
Abstract
We have developed a protocol for rapid generation of barley plants with introduced genes by employing techniques of tissue culture, plant regeneration and crossing. Compared to conventional protocols, the time between pollination of To plants and identification of phenotypic expression in the T(1) generation is reduced from around 17-9 weeks. All of the selected candidates after the tissue culture screening exhibited phenotypic expression. Furthermore, by utilizing ordinary pollen crossing, we demonstrated a route for introduction of genes to a recalcitrant barley elite cultivar. This Tissue Culture after Crossing (TCC) procedure significantly enhances the prospects for producing transgenic barley lines from desirable germplasm. We suggest that the TCC method will contribute to improve barley as a promising bioenergy crop. (C) 2011 Elsevier Ltd. All rights reserved.
Keywords
Barley; Bioenergy; Tissue culture; Transgenics
Published in
Applied Energy
2012, volume: 91, number: 1, pages: 405-411
SLU Authors
Sun, Chuanxin
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Bioenergy
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1016/j.apenergy.2011.09.042
Permanent link to this page (URI)
https://res.slu.se/id/publ/37552