Spider silk proteins and methods for producing spider silk proteins

Abstract

The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

SLU Authors

• Johansson, Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Hjälm, Göran

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Stark, Margareta

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Rising, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Grip, Stefan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Engström, Wilhelm

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Hedhammar, My

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Permanent link to this page (URI)

https://res.slu.se/id/publ/42809