Spider silk fusion protein structures for binding to an organic target

Abstract

A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

SLU Authors

• Hedhammar, My

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Johansson, Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Rising, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Other Basic Medicine

Permanent link to this page (URI)

https://res.slu.se/id/publ/42823