Monomeric N-terminal spider silk protein domain and uses thereof

Abstract

A novel protein is comprising a moiety of 100-160 amino acid residues having at least 80% identity with the N-terminal moiety of a spider silk protein, wherein the amino acid residue corresponding to position 72 in SEQ ID NO 1 is not Ala or Gly. The protein moiety is useful in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide. Novel methods are provided for producing such a fusion protein, and optionally a desired protein or polypeptide from said fusion protein.

SLU Authors

• Johansson, Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Nordling, Kerstin

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Rising, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biomedical Laboratory Science/Technology

Permanent link to this page (URI)

https://res.slu.se/id/publ/44358