Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Abstract

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.

Keywords

Whole blood; RNA preservation; RNA extraction; DNA contamination; Transcriptome sequencing; Globin transcripts

Published in

BMC Biotechnology
2012, volume: 12, pages: 1-11
Publisher: BIOMED CENTRAL LTD

SLU Authors

• Schowochow, Doreen

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology
Other Environmental Biotechnology

Publication identifier

• DOI: https://doi.org/10.1186/1472-6750-12-33

Permanent link to this page (URI)

https://res.slu.se/id/publ/45166