Welander, Margareta
- Department of Crop Science, Swedish University of Agricultural Sciences
Abstract
A highly efficient three-step protocol for in vitro propagation of Ensete ventricosum (enset) was developed that consisted of initiation, bud proliferation, and shoot elongation and rooting stages. At the initiation stage, it was crucial to use shoot tips (5-8 mm) with subtending corm tissues as explants to obtain growth. The addition of 0.5-1% (w/v) activated charcoal to the medium was essential to prevent phenol exudation which otherwise leads to the loss of cultures. During the bud proliferation stage, modified MS macronutrients and micronutrients together with a combination of cytokinins (1.6 muM naphthaleneacetic acid, 4.4 muM 6-benzylaminopurine, 23.2 muM kinetin, 22.6 muM N-6 2-isopentyladenine) was used. This novel composition of macronutrients was based on the analysis of leaf nutrient content of glasshouse-grown enset sprouts. Multiple bud formation on the enlarged corm tissue was induced only when the meristem region was wounded before transfer to the bud proliferation medium. Up to 75 healthy shoots per explant were produced, whereas unwounded explants produced, only one to two shoots per explant. A third stage with a low concentration of cytokinin enabled shoot elongation as well as root development. The plantlets were acclimatized with 100% success and they showed no apparent phenotypical deviation.
Keywords
apical dominance; Ensete ventricosum; micropropagation; Musaceae; wounding
Published in
Plant Cell Reports
2004, volume: 23, number: 5, pages: 277-283
Publisher: SPRINGER
SLU Authors
Birmeta, Genet
- Department of Crop Science, Swedish University of Agricultural Sciences
- Department of Crop Science, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Forest Science
Publication identifier
- DOI: https://doi.org/10.1007/s00299-004-0832-9
Permanent link to this page (URI)
https://res.slu.se/id/publ/4735