Höglund, Anna-Stina
- Department of Cell Research, Swedish University of Agricultural Sciences
Abstract
Immunocytochemical studies on Brassica napus (rapeseed) tissues using a monoclonal antibody against myrosinase thioglucoside glucohydrolase) showed that the enzyme was only present in a small number of cells. In the developing embryo, scattered myrosinase-containing cells were present in both cotyledons and axis. The enzyme accumulated in these cells during the later stages of seed development, approximately from day 20 until day 40 after pollination. Parallel staining with the immunocytochemical technique and a histochemical method identified these cells as myrosin cells. Myrosinase appeared to be located outside the myrosin grains, although the occasional association with the membrane of the grains also was noted. In leaves, petals, and siliques, scattered parenchyma cells were stained in the mesophyll as well as in the vascular tissue. In young leaves, guard cells also contained myrosinase. The enzyme was also present in xylem cells of the stem.
Published in
Plant Physiology
1991, volume: 95, number: 1, pages: 213-221
Publisher: AMER SOC PLANT PHYSIOLOGISTS
SLU Authors
Lenman, Marit
- Department of Cell Research, Swedish University of Agricultural Sciences
- Department of Cell Research, Swedish University of Agricultural Sciences
Falk, Anders
- Department of Cell Research, Swedish University of Agricultural Sciences
- Department of Cell Research, Swedish University of Agricultural Sciences
Rask, Lars
- Department of Cell Research, Swedish University of Agricultural Sciences
- Department of Cell Research, Swedish University of Agricultural Sciences
UKÄ Subject classification
Cell Biology
Publication identifier
- DOI: https://doi.org/10.1104/pp.95.1.213
Permanent link to this page (URI)
https://res.slu.se/id/publ/52305