Munoz, Ines G.
- Department of Molecular Biology, Swedish University of Agricultural Sciences
Abstract
The glycohydrolase family 5 beta-mannanase Man5A from Mytilus edulis has been expressed in Pichia pastoris and purified in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop technique at 293 K using polyethylene glycol 5000 monomethylether as precipitant and dioxane as additive. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.8, b = 64.8, c = 90.7 Angstrom. Diffraction to 1.4 Angstrom resolution has been obtained at 100 K. Expression was also performed in the presence of selenomethionine. The incorporation of SeMet was estimated at 40% by amino-acid analysis and its presence in crystals was confirmed from the X-ray absorption scanning spectrum.
Published in
Acta Crystallographica Section D: Biological Crystallography
2002, volume: 58, pages: 542-545
Publisher: BLACKWELL MUNKSGAARD
SLU Authors
Ståhlberg, Jerry
- Department of Molecular Biology, Swedish University of Agricultural Sciences
- Department of Molecular Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Structural Biology
Publication identifier
- DOI: https://doi.org/10.1107/S0907444902000355
Permanent link to this page (URI)
https://res.slu.se/id/publ/53155