Hilden, Lars
- Department of Forest Products, Swedish University of Agricultural Sciences
Abstract
The alpha-amino group of the carbohydrate-binding module (CBM) from Phanerochaete chrysosporium cellulase Cel7D was covalently labelled with fluorescein isothiocyanate. The fluorescein-labelled CBM was characterised regarding substrate binding, showing specificity only to cellulose and not to mannan and xylan. Conjugation of fluorescein isothiocyanate to CBM did not affect its binding to cellulose. The labelled CBM was successfully used as a probe for detecting cellulose in lignocellulose material such as never dried spruce and birch wood as well as pulp fibres
Published in
Biotechnology Letters
2003, volume: 25, number: 7, pages: 553-558
Publisher: KLUWER ACADEMIC PUBL
SLU Authors
Daniel, Geoffrey
- Department of Forest Products, Swedish University of Agricultural Sciences
- Department of Forest Products, Swedish University of Agricultural Sciences
UKÄ Subject classification
Forest Science
Publication identifier
- DOI: https://doi.org/10.1023/A:1022846304826
Permanent link to this page (URI)
https://res.slu.se/id/publ/542