Cytokine induction by immunostimulatory DNA in porcine PBMC is impaired by a hairpin forming sequence motif from the genome of Porcine Circovirus type 2 (PCV2)

Abstract

The effect of ODN PCV2/1 was studied further by monitoring the expression of mRNA for IFN-alpha, IL-12p40, IL-10, IL-6, IFN-gamma, IL-1 beta, TGF-beta, and TNF-alpha in cultures of poPBMC stimulated with ODN 2216 or Poly I:C. Results from qPCR analyses showed that ODN PCV2/1 clearly inhibited the expression of IFN-alpha, IL-12p40, IL-10 and IL-6 when induced by ODN 2216, but did not seem to affect any of the cytokines examined when induced by Poly I:C. Initial studies using confocal microscopy and fluorochrome labelled ODNs indicate that ODN 2216 and ODN PCV2/1 co-localize in subpopulations of poPBMC. (C) 2010 Elsevier B.V. All rights reserved.

Keywords

IFN-alpha; PCV2; Cytokine mRNA; Inhibitory ODN

Published in

Veterinary Immunology and Immunopathology
2011, volume: 139, number: 2-4, pages: 156-166
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Hasslung Wikström, Frida

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Fossum, Caroline

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Fuxler, Lisbeth

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Kruse, Robert

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1016/j.vetimm.2010.09.010

Permanent link to this page (URI)

https://res.slu.se/id/publ/57052