Skip to main content
SLU publication database (SLUpub)

Abstract

More than 100 accessions of Hordeum marinum ssp. marinum, ssp. gussoneanum 2x and ssp. gussoneanum 4x were studied by analyzing the nuclear DNA homologous to the chloroplast translation elongation factor. PCR amplification using the locus-specific primers generated a 932 bp fragment from ssp. marinum, a 915 bp fragment from ssp. gussoneanum 2x, and 915 bp and 931 bp fragments from ssp. gussoneanum 4x. Sequencing of the entire DNAs showed that the 915-bp fragments of diploids and tetraploids are identical, indicating that the diploid probably is the immediate donor of the 915-bp fragment of tetraploids. Although the donor of the 931-bp fragment was not determined, phylogenetic analysis of the data showed that the clade of the 931-bp fragment was included in the H. marinum complex. A MspI site was diagnostic to distinguish ssp. marinum into two clades with disjunct distributions, the Iberian Peninsula (type 2) and other regions (type 1). Nucleotide variation was revealed in the type 2 accessions but all the other clades were highly uniform.

Keywords

Triticeae; Poaceae; phylogeny; chloroplast translation elongation factor; DNA sequences; maximum parsimony analysis; cladistic analysis; polyploidy; genome; spike morphology

Published in

Plant Systematics and Evolution
2001, volume: 227, number: 45720, pages: 137-144
Publisher: SPRINGER-VERLAG WIEN

SLU Authors

  • Salomon, Björn

    • Department of Crop Science, Swedish University of Agricultural Sciences

  • Bryngelsson, Tomas

    • Department of Crop Science, Swedish University of Agricultural Sciences

UKÄ Subject classification

Agricultural Science

Publication identifier

  • DOI: https://doi.org/10.1007/s006060170044

Permanent link to this page (URI)

https://res.slu.se/id/publ/5892