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Abstract

The objectives were to determine the 1) extent of epithelial and red blood cell contamination in epididymal cat sperm samples recovered by the cutting method, 2) efficacy of simple washing, single-layer centrifugation (SLC), and swim-up for selecting epididymal cat sperm, and 3) effects of freezing and thawing on cat sperm selected by various techniques Ten unit samples were studied, each contained sperm from the cauda epididymides of four cats (total, similar to 200 x 10(6) sperm) and was equally allocated into four treatments 1) simple washing. 2) single-layer centrifugation through colloid prior to cryopreservation (SLC-PC), 3) single-layer centrifugation through colloid after cryopreservation (SLC-AC), and 4) swim-up Centrifugation (300 x g for 20 min) was done for all methods The SLC-PC had a better recovery rate than the SLC-AC and swim-up methods (mean +/- SD of 16 4 +/- 8 7, 107 +/- 8 9, and 2.3 +/- 1 7%, respectively. P < 0 05). The SLC-PC. SLC-AC and swim-up samples contained less red blood cell contamination than simple washed samples (0 02 +/- 0 01, 0 02 +/- 0 04, 0 03 +/- 0.04, and 0 44 +/- 0 22 x 10(6) cells/mL. respectively; P < 0 05) Although the proportion of sperm with head abnormalities did not differ among selection methods (P > 0 05), SLC-PC yielded the highest percentage of sperm with normal midpieces and tails (P < 0 05), due to the lowest proportion of coiled tails (P < 0 05) Furthermore, the SLC-PC was as effective as swim-up in removing sperm with proximal droplets, and selecting motile sperm, as well as those with intact membranes and DNA (P > 0 05) In conclusion. both SLC-PC and swim-up improved the quality of epididymal cat sperm, including better morphology, membrane and DNA integrity, and removal of cellular contamination However, SLC had a better sperm recovery rate than swim-up (c) 2010 Elsevier Inc. All rights reserved

Keywords

Colloid; Sperm separation; Semen; Sperm morphology; Feline

Published in

Theriogenology
2010, volume: 73, number: 9, pages: 1284-1292
Publisher: ELSEVIER SCIENCE INC

SLU Authors

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science

Publication identifier

  • DOI: https://doi.org/10.1016/j.theriogenology.2009.12.009

Permanent link to this page (URI)

https://res.slu.se/id/publ/60494