Phylogenetic analysis of the genus Sorghum based on combined sequence data from cpDNA regions and ITS generate well-supported trees with two major lineages

Abstract

Background and Aims Wild Sorghum species provide novel traits for both biotic and abiotic stress resistance and yield for the improvement of cultivated sorghum. A better understanding of the phylogeny in the genus Sorghum will enhance use of the valuable agronomic traits found in wild sorghum.Methods Four regions of chloroplast DNA (cpDNA; psbZ-trnG, trnY-drnD, trnY-psbM and trnT-trnL) and the internal transcribed spacer (ITS) of nuclear ribosomal DNA were used to analyse the phylogeny of sorghum based on maximum-parsimony analyses.Key Results Parsimony analyses of the ITS and cpDNA regions as separate or combined sequence datasets formed trees with strong bootstrap support with two lineages: the Eu-sorghum species S. laxiflorum and S. macrospermum in one and Stiposorghum and Para-sorghum in the other. Within Eu-sorghum. S. bicolor-3, -11 and -14 originating from southern Africa form a distinct clade. S. bicolor-2, originally from Yemen, is distantly related to other S. bicolor accessions.Conclusions Eu-sorghum species are more closely related to S. macrospemum and S. laxiflorum than to any other Australian wild Sorghum species. S. macrospermum and S. laxiflorum are so closely related that it is inappropriate to classify them in separate sections. S. oilman is closely associated with S. bicolor, suggesting that the latter is the maternal parent of the former given that cpDNA is maternally inherited in angiosperms. S. bicolor-3. -11 and -14, from southern Africa, are closely related to each other but distantly related to S. bicolor-2.

Keywords

Molecular phylogeny; Sorghum; Eu-sorghum; Zea mays; non-coding regions; cpDNA; ITS

Published in

Annals of Botany
2010, volume: 105, number: 3, pages: 471-480
Publisher: OXFORD UNIV PRESS

SLU Authors

• Ng´uni, Dickson

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Dida, Mulatu Geleta

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Fatih, Moneim

  • Department of Urban and Rural Development, Swedish University of Agricultural Sciences
    

• Bryngelsson, Tomas

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Swedish Biodiversity Centre

UKÄ Subject classification

Food Science
Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1093/aob/mcp305

Permanent link to this page (URI)

https://res.slu.se/id/publ/60811