Heneen, Waheeb
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Abstract
Different approaches have been applied to characterize the A and C genomes of Brassica rapa and B. oleracea, respectively. One approach was the morphological differentiation of karyotyped chromosomes (Cheng et al., Genome 38: 313-319, 1995). This was further facilitated by the use of florescence and genomic in-situ hybridization (FISH and GISH) applying ribosomal and other DNA probes, benefitting from the advances made on the molecular DNA characterization of these genomes. A second approach was combining the gained molecular knowledge with genetic inheritance analysis, thereby defining the different linkage groups of DNA markers. Cytologic and genetic approaches lead to independent numerical designations of the chromosomes and linkage groups. Knowledge of the specificity of certain DNA probe/marker to a specific chromosome or genome and to a specific linkage group is a key to disclose the correspondence between these different designations. This culminated in assigning all linkage groups to identifiable A- and C-genome chromosomes that were given the same numerical designations of the linkage groups (Xiong and Pires, genetics 187: 37-49, 2011). These numerals were different from those adopted in the karyotype analysis (Cheng et al.) based on cytological criteria. The cytological designations were adopted when a series of nine monosomic addition lines of B. rapa carrying each of the nine different chromosomes of B. oleracea were developed. These were characterized by FISH and GISH probes that are C-chromosome or C-genome specific, and also by simple sequence repeat (SSR) DNA markers that are C-chromosome (addition line) specific and/or C-genome linkage group specific (Heneen et al., Ann. Bot. 109, 1227-1242, 2012; Geleta et al., Theor. Appl. Genet. 125: 455-466, 2012). In that way, the correspondence between the nine karyotyped chromosomes of the C-genome and their linkage groups was determined. Continued such efforts may contribute to a future unified designation system.
Published in
Title: The 19th Crucifer Genetic Workshop and Brassica 2014 - “Genetic Improvement of Brassicaceae Crops in the Era of Genomics”
SLU Authors
Dida, Mulatu Geleta
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
UKÄ Subject classification
Genetics and Breeding in Agricultural Sciences
Permanent link to this page (URI)
https://res.slu.se/id/publ/66729