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Abstract

Finnsheep is one of the most prolific sheep breeds in the world. We sequenced RNA-Seq libraries from the ovaries of Finnsheep ewes collected during out of season breeding period at about 30X sequence coverage. A total of 86966348 and 105587994 reads from two samples were mapped against latest available ovine reference genome (Oarv3.1). The transcriptome assembly revealed 14870 known ovine genes, including the 15 candidate genes for fertility and out-of-season breeding. In this study we successfully used our bioinformatics pipeline to assemble the first ovarian transcriptome of Finnsheep.

Keywords

bioinformatics pipeline; mRNA; prolific sheep breed

Published in

Agricultural and Food Science
2015, volume: 24, number: 1, pages: 1-9

SLU Authors

UKÄ Subject classification

Genetics and Breeding in Agricultural Sciences

Publication identifier

  • DOI: https://doi.org/10.23986/afsci.46512

Permanent link to this page (URI)

https://res.slu.se/id/publ/68036