Skip to main content
SLU publication database (SLUpub)

Abstract

The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Articular cartilage explants were stimulated with interleukin (IL)-1 beta in vitro for 25 days, and proteins released into cell culture media were chemically labeled with isobaric mass tags and analyzed by liquid chromatography-tandem mass spectrometry. A total of 127 proteins were identified and quantified in media from explants. IL-1 beta-stimulation resulted in an abundance of proteins related to inflammation, including matrix metalloproteinases, acute phase proteins, complement components and IL-6. Extracellular matrix (ECM) molecules were released at different time points, and fragmentation of aggrecan and cartilage oligomeric matrix protein was observed at days 3 and 6, similar to early-stage OA in vivo. Degradation products of the collagenous network were observed at days 18 and 22, similar to late-stage OA. This model displays a longitudinal quantification of released molecules from the ECM of articular cartilage. Identification of dynamic changes of extracellular matrix molecules in the secretome of equine explants stimulated with IL-1 beta over time may be useful for identifying components released at different time points during the spontaneous OA process.

Keywords

Horse; IL-1 beta; matrix molecules; osteoarthritis; proteomics

Published in

Connective Tissue Research
2015, volume: 56, number: 4, pages: 315-325
Publisher: INFORMA HEALTHCARE

SLU Authors

UKÄ Subject classification

Cell and Molecular Biology

Publication identifier

  • DOI: https://doi.org/10.3109/03008207.2015.1027340

Permanent link to this page (URI)

https://res.slu.se/id/publ/68415