Zmihorski, Michal
- Department of Ecology, Swedish University of Agricultural Sciences
- Polish Academy of Sciences (PAN)
Abstract
We present an optimized multiplex of 14 microsatellite loci that can be amplified in four reactions and loaded as two separate panels on the ABI genetic analyzer. The assay was validated on 342 individuals of field cricket (Gryllus campestris, L., 1758) from natural populations. The chosen loci are polymorphic and allow for efficient population genetic analysis, although we observed some aberrations, which are discussed. The optimized multiplex sets show reproducible amplification pattern with low genotyping error rate and should be therefore very useful in analyses of both experimental as well as natural populations of crickets. The optimized multiplex outperforms previously developed sets as it allows for amplification of 14 loci in four instead of seven reactions. These panels may serve as an applicable tool in conservation programs in countries in which this species is endangered. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
Keywords
Gryllus campestris; Microsatellite loci; Multiplex PCR; Landscape genetics; Species conservation
Published in
Journal of Asia-Pacific Entomology
2015, volume: 18, number: 3, pages: 421-424
Publisher: KOREAN SOC APPLIED ENTOMOLOGY
SLU Authors
UKÄ Subject classification
Genetics and Genomics
Publication identifier
- DOI: https://doi.org/10.1016/j.aspen.2015.04.009
Permanent link to this page (URI)
https://res.slu.se/id/publ/70098