Histological analysis reveals the formation of shoots rather than embryos in regenerating cultures of Eucalyptus globulus

Abstract

Eucalyptus globulus is an important species in international forestry in regions with Mediterranean climates and comprises 65 % of the plantation hardwood in Australia. Propagation by somatic embryogenesis would offer many advantages and its development has received much attention. Structures regenerating on explants from hypocotyls of mature zygotic embryos of E. globulus cultured on medium with NAA, reported previously to be effective for embryogenic regeneration, were analyzed morphologically and histologically to clarify their pathway of development. Analysis of series of sections revealed organogenic, rather than embryogenic, pathways of regeneration in this system. We show that protocols for propagation of E. globulus must be carefully evaluated by microscopic examination of adequate numbers of serial sections.

Keywords

Eucalyptus globulus; Histological studies; In vitro culture; Regeneration

Published in

Plant Cell, Tissue and Organ Culture
2017, volume: 128, number: 2, pages: 319-326

SLU Authors

• Dobrowolska, Izabela

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • University of Silesia

• Andrade, Gisele

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Clapham, David

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Egertsdotter, Ulrika

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Georgia Institute of Technology

Global goals (SDG)

SDG15 Life on land

UKÄ Subject classification

Developmental Biology

Publication identifier

• DOI: https://doi.org/10.1007/s11240-016-1111-5

Permanent link to this page (URI)

https://res.slu.se/id/publ/77921