Nilsson, Thomas
- Department of Forest Products, Swedish University of Agricultural Sciences
Abstract
This paper describes procedures used in attempts to isolate pure strains of erosion bacteria from bacterial consortia obtained from decaying wood. The wood originated from archaeological waterlogged wood and foundation pilings. Kapok fibres were used as a substitute for wood in most experiments. Erosion bacteria attached firmly to the surface of kapok fibres and to cellophane. Intense washing of these substrates resulted in active cultures with a reduced number of associated secondary bacteria. This was not achieved with common dilution techniques. Pure strains obtained from streaking on cellulose or nutrient agar failed to attack kapok fibres and pine wood. Single erosion bacteria cells were separated from secondary bacteria by using laser techniques (optical tweezers). Molecular analyses of the most purified culture, obtained through washing of cellophane, and of laser separated bacteria, suggested that erosion bacteria belong to the CFB (Cytophaga-Flavobacterium-Bacteroides) complex. (C) 2007 Elsevier Ltd. All rights reserved.
Keywords
archaeological wood; cellophane; decay; erosion bacteria; foundation pilings; isolation; kapok fibres; laser techniques; micro-dissection; optical tweezers; wood
Published in
International Biodeterioration and Biodegradation
2008, volume: 61, number: 1, pages: 17-23
Publisher: ELSEVIER SCI LTD
SLU Authors
Björdal Gjelstrup, Charlotte
- Department of Forest Products, Swedish University of Agricultural Sciences
- Department of Forest Products, Swedish University of Agricultural Sciences
UKÄ Subject classification
Wood Science
Publication identifier
- DOI: https://doi.org/10.1016/j.ibiod.2007.06.010
Permanent link to this page (URI)
https://res.slu.se/id/publ/77942