Doyle, Siamsa
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Book chapter2014
Using a Reverse Genetics Approach to Investigate Small-Molecule Activity
Doyle, Siamsa; Robert, Stephanie
Abstract
Chemical genomics is a highly effective approach for understanding complex and dynamic biologicalprocesses in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a hugeabundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here wepresent an approach to characterize a chemical of interest, as well as examples of studies demonstrating aneffective combination of chemical genomics with reverse genetics strategies, drawn from recent literatureon phytohormone signalling and auxin transport.
Keywords
Chemical genomics; Arabidopsis mutants; Reverse genetics; Phytohormone signalling; Auxin transport
Published in
Methods in Molecular Biology
2014, volume: 1056, number: 1056, pages: 51-62
Title: Plant chemical genomics : methods and protocols
Publisher: Springer
SLU Authors
Robert, Stephanie
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Biochemistry and Molecular Biology
Botany
Developmental Biology
Publication identifier
- DOI: https://doi.org/10.1007/978-1-62703-592-7_6
- ISBN: 978-1-62703-591-0
- eISBN: 978-1-62703-592-7
Permanent link to this page (URI)
https://res.slu.se/id/publ/79515