Development of Combining of Human Bronchial Mucosa Models with XposeALI (R) for Exposure of Air Pollution Nanoparticles

Abstract

BackgroundExposure to agents via inhalation is of great concerns both in workplace environment and in the daily contact with particles in the ambient air. Reliable human airway exposure systems will most likely replace animal experiment in future toxicity assessment studies of inhaled agents.MethodsIn this study, we successfully established a combination of an exposure system (XposeALI) with 3D models mimicking both healthy and chronic bronchitis-like mucosa by co-culturing human primary bronchial epithelial cells (PBEC) and fibroblast at air-liquid interface (ALI). Light-, confocal microscopy, scanning-and transmission electron microscopy, transepithelial electrical resistance (TEER) measurement and RT-PCR were performed to identify how the PBEC differentiated under ALI culture condition. Both models were exposed to palladium (Pd) nanoparticles which sized 6 - 10 nm, analogous to those released from modern car catalysts, at three different concentrations utilizing the XposeALI module of the PreciseInhale (R) exposure system.ResultsExposing the 3D models to Pd nanoparticles induced increased secretion of IL-8, yet the chronic bronchitis-like model released significantly more IL-8 than the normal model. The levels of IL-8 in basal medium (BM) and apical lavage medium (AM) were in the same ranges, but the secretion of MMP-9 was significantly higher in the AM compared to the BM.ConclusionThis combination of relevant human bronchial mucosa models and sophisticated exposure system can mimic in vivo conditions and serve as a useful alternative animal testing tool

Published in

PLoS ONE
2017, volume: 12, number: 1, article number: e0170428
Publisher: PUBLIC LIBRARY SCIENCE

SLU Authors

• Kessler, Vadim

  • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
    

• Seisenbaeva, Gulaim

  • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Materials Chemistry
Inorganic Chemistry
Environmental Health and Occupational Health

Publication identifier

• DOI: https://doi.org/10.1371/journal.pone.0170428

Permanent link to this page (URI)

https://res.slu.se/id/publ/80504