Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Abstract
This study compared the efficacy of simple sperm washing (SW), single-layer centrifugation (SLC) and modified swim-up (SU) techniques in the preparation of dog spermatozoa for cooling. Eighteen ejaculates, collected from three dogs (six per dog), were pooled (three ejaculates per pool) and divided into three aliquots: (1) one aliquot was washed and cooled at 5 degrees C for 72h, considered as control (SW-control), (2) the second aliquot was selected by SLC through Androcoll-C and subsequently cooled in the same way as the SW-control samples (SLC-AC) and (3) the last aliquot was selected by a modified SU method with Androcoll-C and cooled as mentioned above (SU-AC). Assessment of sperm motility, sperm morphology, sperm membrane integrity and acrosome integrity were performed on aliquots of fresh semen and chilled-rewarmed samples. Sperm membrane integrity and progressive motility were significantly (P0.05) different between SW-control, SLC-AC and SU-AC samples. Our results confirm that SU-AC may be a successful method for the preparation of dog spermatozoa for cooling.
Keywords
Androcoll-C; chilled dog semen; colloid; sperm migration; sperm selection
Published in
Reproduction, Fertility and Development
2016, volume: 28, number: 10, pages: 1545-1552
Publisher: CSIRO PUBLISHING
SLU Authors
UKÄ Subject classification
Developmental Biology
Publication identifier
- DOI: https://doi.org/10.1071/RD15071
Permanent link to this page (URI)
https://res.slu.se/id/publ/82760