Dvorák Tomastíková, Eva
- Palacký University Olomouc
Abstract
BACKGROUND. Steroid receptor coactivators p300 and CBP are highly expressed in advanced prostate cancer. They potentiate activation of androgen receptor by androgens and anti-androgens. In the present study, we have addressed the question whether these coactivators enhance activity of estrogen receptor-beta (ER-beta), which is variably expressed in prostate cancers. METHODS. Expression levels of the coactivators p300 and CBP were manipulated by plasmid or siRNA transfections and activity of ER-beta was measured by luciferase assays. Viability was measured by MTT assays and cellular migration was determined by wound-healing and Boyden chamber assays. RESULTS. High expression of ER-beta was found in PC3 cells which were used for the experiments. p300 or CBP enhanced activation of ER-beta by genistein. Antiestrogens did not acquire agonistic properties in the presence of increased concentrations of either coactivator. Inhibition of p300 or CBP decreased genistein stimulation of ER-beta. Genistein reduced migration of PC3 prostate cancer cells and down-regulation of p300 potentiated this effect. CONCLUSIONS. p300 and CBP are implicated in regulation of ER-beta activity and cellular migration in prostate cancer. These findings are important for understanding of action of ER-beta in carcinoma of the prostate. Prostate 71: 431-437, 2011. (C) 2010 Wiley-Liss, Inc.
Keywords
transcriptional coactivators; prostate cancer; estrogen receptor-beta; migration; genistein
Published in
Prostate
2011, volume: 71, number: 4, pages: 431-437
Publisher: WILEY-BLACKWELL
SLU Authors
UKÄ Subject classification
Clinical Medicine
Publication identifier
- DOI: https://doi.org/10.1002/pros.21257
Permanent link to this page (URI)
https://res.slu.se/id/publ/85597