Üstün, Suayib
- University of Erlangen-Nuremberg
Abstract
Type III effector proteins (T3Es) of many Gram-negative pathogenic bacteria manipulate highly conserved cellular processes, indicating conservation in virulence mechanisms during the infection of hosts of divergent evolutionary origin. In order to identify conserved effector functions, we used a cross-kingdom approach in which we expressed selected T3Es from the mammalian pathogen Salmonella enterica in leaves of Nicotiana benthamiana and searched for possible virulence or avirulence phenotypes. We show that the T3E SseF of S. enterica triggers hypersensitive response (HR)-like symptoms, a hallmark of effector-triggered immunity in plants, either when transiently expressed in leaves of N similar to benthamiana by Agrobacterium tumefaciens infiltration or when delivered by Xanthomonas campestris pv vesicatoria (Xcv) through the type III secretion system. The ability of SseF to elicit HR-like symptoms was lost upon silencing of suppressor of G2 allele of skp1 (SGT1), indicating that the S. enterica T3E is probably recognized by an R protein in N similar to benthamiana. Xcv translocating an AvrRpt2SseF fusion protein was restricted in multiplication within leaves of N similar to benthamiana. Bacterial growth was not impaired but symptom development was rather accelerated in a compatible interaction with susceptible pepper (Capsicum annuum) plants. We conclude that the S. enterica T3E SseF is probably recognized by the plant immune system in N similar to benthamiana, resulting in effector-triggered immunity.
Keywords
hypersensitive response (HR); plant-bacteria interaction; R protein; Salmonella; SseF; type III effector protein (T3E)
Published in
NEW PHYTOLOGIST
2012, volume: 194, number: 4, pages: 1046-1060
Publisher: WILEY-BLACKWELL
SLU Authors
UKÄ Subject classification
Biochemistry
Molecular Biology
Publication identifier
- DOI: https://doi.org/10.1111/j.1469-8137.2012.04124.x
Permanent link to this page (URI)
https://res.slu.se/id/publ/87529